| CatalogCode: | NB110-57027 |
| ProductName: | H-Ras (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-H-Ras (C-term) (Y132) |
| Clone: | Y132 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic peptide corresponding to residues in the C-terminus of human H-Ras. |
| Specificity: | A synthetic peptide corresponding to residues in the C-terminus of human H-Ras was used as an immunogen. The antibody does not cross-react with other Ras family member. |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml protein A purified Rabbit ascites. |
| Uses: | WB: 1:500ICC: 1:100-250IP: 1:50 |
| Background: | Ras, a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-c H ras 1 antibody, anti-c H ras 1 protein antibody, anti-c H ras antibody, anti-c has/bas p21 protein antibody, anti-c ras Ki 2 protein antibody, anti-cHras antibody, anti-G1III6 N ras antibody, anti-GTPase HRas antibody, anti-H Ras 1 antibody, anti-H ras antibody, anti-H RasIDX antibody, anti-Ha ras antibody, anti-Harvey ras antibody, anti-Harvey rat sarcoma virus oncogene 1 antibody, anti-Hras 1 antibody, anti-HRAS antibody, anti-Hras1 antibody, anti-K ras antibody, anti-N ras antibody, anti-N ras oncogene antibody, anti-P19 H RasIDX protein antibody, anti-p21 protein antibody, anti-p21ras antibody, anti-RASH 1 antibody, anti-RASH1 antibody, anti-Transforming protein p21 antibody, anti-v Ha ras Harvey rat sarcoma viral oncogene homolog antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Ellis RW, et al. Nature. 1981 Aug 6;292(5823):506-511 2. Campbell, S. L., (1998) Oncogene 17, 1395-1413 3. Zheng, Y., and Quilliam, L. A. (2003) EMBO Rep 4, 463-468 4. Bos JL. Cancer Res. 1989;49:4682-4689 |
|