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| Catalog Code: | NB110-57015 |
| Product Name: | GRP1 Antibody |
| Product Description: | Rabbit Monoclonal anti-GRP1 (EP394Y) |
| Clone: | EP394Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues near the SEC7 domain of GRP1 was used as immunogen. |
| Cross Reactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500 |
| Background: | GRP1 (general receptor for 3-phosphoinositides) is composed of an N-terminal heptad repeat, an Arf exchange domain with homology to the yeast Sec7 protein, a PH domain, and a C-terminal polybasic region (1). The PH domain of GRP1 binds PtdIns(3, 4, 5)P3 with high affinity, but not PtdIns(3, 4)P2 or PtdIns(3)P, potentially making it a target for PI-3-kinase. Furthermore, GRP1 acts as a nucleotide exchange factor for the small GTP proteins of the ARF family (2). While GRP1 catalyses guanine-nucleotide exchange specifically on ARF5; other GRP1 homologues (ARNO and cytohesin-1) will only function on ARF6 and ARF3 respectively (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-GRASP antibody, anti-GRP1 associated scaffold protein antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Klarlund J, Guilherme A, Holik JJ, Virbasius J, V, Chawla A, Czech MP 1997 Science 275:1927-1930 2. Klarlund et al. J Biol Chem 273:1859-1864 3. Klarlund, J. K., Rameh, L. E., Cantley, L. C.,Buxton, J. M., Holik, J. J., Sakelis, C., Patki, V., Corvera, S. and Czech,M. P. (1998) J. Biol. Chem. 273, 1859-1862 |
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