| CatalogCode: | NB110-56999 |
| ProductName: | Gab1 Antibody |
| Product Description: | Rabbit Monoclonal anti-Gab1 (Y423) |
| Clone: | Y423 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues in human Gab1 was used as immunogen. The antibody is predicted to detect splice isoform 2 based on sequence analysis. |
| CrossReactivity: | Reacts in human, mouse, and rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000-5,000ICC: 1:50 |
| Background: | Gab1 (Grb2-associated binder-1) is an adaptor protein that serves to activate numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs). Gab1 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors, and B cell antigen receptors; and it acts as a docking protein for several SH2-containing proteins (1-3). Tyrosine-phosphorylated Gab1 may recruit cytosolic signaling proteins to the cellular membrane, where they can act on membrane-bound targets as well as activate downstream pathways (4) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-Gab1 antibody, anti-GRB 2 associated binder 1 antibody, anti-GRB 2 associated binding protein 1 antibody, anti-GRB2 associated binder 1 antibody, anti-GRB2 associated binding protein 1 antibody, anti-GRB2 associated binding protein 1 isoform a antibody, anti-GRB2 associated binding protein 1 isoform b antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Holgado-Madruga, M., et al. A Grb2-associated docking protein in EGF- and insulin-receptor signalling. Nature 379: 560-4 (1996). 2. Ingham, R.J., et al. The Gab1 docking protein links the b cell antigen receptor to the phosphatidylinositol 3-kinase/Akt signaling pathway and to the SHP2 tyrosine phosphatase. J. Biol. Chem. 276: 12257-12265 (2001). 3. Ingham, R.J., et al. The Gab1 protein is a docking site for multiple proteins involved in signaling by the B cell antigen receptor. J Biol Chem. 273: 30630-7 (1998). 4. Schaeper, U., et al. Coupling of Gab1 to c-Met, Grb2, and Shp2 mediates biological responses. J Cell Biol. 149: 1419-32 (2000). |
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