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| Catalog Code: | NB110-56976 |
| Product Name: | Ezrin Antibody |
| Product Description: | Rabbit Monoclonal anti-Ezrin (EP886Y) |
| Clone: | EP886Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues near the C-terminus of Human Ezrin. |
| Specificity: | This antibody is specific for Ezrin. |
| Cross Reactivity: | This antibody reacts with the human, mouse, and rat. Other species have not yet been tested. |
| Packaging: | 0.1 ml IgG purified Rabbit ascites. |
| Uses: | The recommended uses for this antibody are Immunocytochemistry/Immunofluorescence, Immunohistochemistry (Paraffin-embedded sections), Immunoprecipitation,Flow Cytometry, Western blot (Detects a band of approximately 72 kDa predicted molecular weight: 69 kDa). |
| Background: | Erzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Ezrin activity is regulated by intramolecular interactions between N- and C-terminal ERM association domains (3). Phosphorylation at threonine 567 is a critical regulator of ezrin function allowing the active protein to link target molecules to the actin cytoskeleton (4). Erzin tyrosine phosphorylation can also be induced by EGF, PDGF and HGF stimulation. Erzin interacts with PI3-K protein kinase A and Rho (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 |
| Application Summary: | flow cytometry / FACS analysis 1/80, immunohistochemistry 1/100 - 1/250, immunofluorescence 1/50 - 1/100, immunoprecipitation 1/50, Western Blot 1/5000 - 1/50000, Immunocytochemistry 1/50 - 1/100 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CVL antibody, anti-Cytovillin antibody, anti-DKFZp762H157 antibody, anti-FLJ26216 antibody, anti-MGC1584 antibody, anti-p81 antibody, anti-VIL 2 antibody, anti-VIL2 antibody, anti-Villin 2 antibody, anti-Villin2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% Sodium Azide |
| General References: | 1. Bretscher, A., Edwards, K., and Fehon, R. G. (2002) Nat. Rev. Mol. Cell. Biol. 3, 586-599 2. Tsukita, S., and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510 3. Pearson, M. A., Reczek, D., Bretscher, A., and Karplus, P. A. (2000) Cell 101, 259-270 4. Gautreau, A., Louvard, D., and Arpin, M. (2000) J. Cell Biol. 150, 193-203 5. Bretscher, A., Chambers, D., Nguyen, R., and Reczek, D. (2000) Annu. Rev. Cell Dev. Biol. 16, 113-143 |
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