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| Catalog Code: | NB110-56961 |
| Product Name: | ER alpha Antibody |
| Product Description: | Rabbit Monoclonal anti-ER alpha (E115) |
| Clone: | E115 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues surrounding Ser104 and Ser106 of human ER alpha was used as immunogen. |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500 IHC: 1:50-1:100 ICC: 1:100 |
| Background: | Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha (2). Particularly, phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-DKFZp686N23123 antibody, anti-ER alpha antibody, anti-ER antibody, anti-Era antibody, anti-ESR 1 antibody, anti-ESR antibody, anti-ESR1 antibody, anti-ESRA antibody, anti-Estradiol receptor antibody, anti-Estrogen receptor 1 antibody, anti-Estrogen receptor antibody, anti-Major ORF antibody, anti-NR3A1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Pakdel, F., et al. Identification of charged residues in an N-terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 7: 1408-17 (1993). 2. Sheeler, C.Q., et al. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 29: 237-55 (2003). 3. Rogatsky, I., et al. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 274: 22296-302 (1999). |
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