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| Catalog Code: | NB110-56957 |
| Product Name: | EphA3 Antibody |
| Product Description: | Rabbit Monoclonal anti-EphA3 (EP725Y) |
| Clone: | EP725Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to the N-term region of Human EphA3 was used as an immunogen. |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB; 1:2000 IP: 1:50 |
| Background: | Ephrin receptor tyrosine kinases (RTKs) and their ligands (ephrins) are highly conserved families which mediates cell movement during development (1). There are 14 known receptors, classed into either EphA or EphB, and 8 known ligands, classed into ephrin A or ephrin B. EphA receptors preferably interacts with glycosylphosphatidylinositol-linked ephrins (ephrin A), while EphB receptors preferably interacts with transmembrane ligands (ephrin B) (2). Multiple ligands are specific to each Ephrin receptors and cause receptor activation through transphosphorylation (3, 4). Both receptor and ligand-initiated signals can regulate repulsion, adhesion and de-adhesion mechanisms for the motility of adherent cells (5). Ephrin A3 (EphA3, Mek4, Hek, Hek4) binds to ephrin-A1 to A5 and ephrin-B1. A somatic mutation on EphA3 gene has been linked to pathogenesis to several tumors, such as breast, lung, and pancreatic cancers (6). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl, 40% Glycerol, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BS |
| Application Summary: | immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Package Size: | 0.1 ml |
| Preservative: | 0.25% sodium azide |
| General References: | 1. Lackmann M et al. J Biol Chem. 273(32):20228-37, 1998. 2. The Eph Nomenclature Committee. (1997) Cell 90, 403-404 3. Beckmann MP et al. EMBO J. 13, 3757-3762, 1994. 4. Pandey A et al J. Biol. Chem. 269, 30154-30157, 1994. 5. Lawerson ID et al. Journal of Cell Science 115, 1059-1072, 2002. 6. Wood LD et al. Hum Mutat. 27(10):1060-1, 2006. |
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