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| Catalog Code: | NB110-56953 |
| Product Name: | Elk-1 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-Elk-1 (C-term) (Y77) |
| Clone: | Y77 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues in C terminus of human ELK1. |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml protein A purified Rabbit ascites. |
| Uses: | WB: 1:500 IHC: 1:50 ICC: 1:100 IP: 1:80 |
| Background: | Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors (2). Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry , immunoprecipitation , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-ELK 1 antibody, anti-ELK1 member of ETS oncogene family antibody, anti-ELK1 protein antibody, anti-ELK2 member of ETS oncogene family antibody, anti-ETS domain containing protein Elk 1 antibody, anti-ETS domain containing protein Elk1 antibody, anti-ETS domain protein Elk1 antibody, anti-Oncogene Elk1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Rao, V.N., et al. elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints. Science 244: 66-70 (1989). 2. Marais, R., et al. The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain. Cell 73: 381-393 (1993). 3. Harindranath, N., et al. The human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the IgH locus. Gene 221: 215-24 (1998). 4. Gille, H., et al. ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J. 14: 951-62 (1995). |
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