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| Catalog Code: | NB110-56949 |
| Product Name: | elF-2alpha Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-elF-2alpha (pS51) (E90) [Ser51] |
| Clone: | E90 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a. |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2á was used as immunogen. The antibody only detects eIF-2á phosphorylated on Serine 51. Predicted to cross-react with bovine and pig, based on sequence homology. |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit ascites. |
| Uses: | WB: 1:500-1,000 IHC: 1:50-100 ICC: 1:100-250 |
| Background: | IRE1 acts as the sensor of unfolded proteins in the ER that initiates transmittance of the unfolded protein signal from the ER to the nucleus by splicing XBP1 mRNA converting it into a potent unfolded-protein response transcriptional activator. It is a transmembrane protein that has both serine-threonine kinase and endoribonuclease activities. Null mutants are viable, but IRE1 is essential for viability under stress conditions that cause unfolded proteins to accumulate in the ER. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry 1:50-1:100, Western Blot 1:500-1:1000, Immunocytochemistry 1:100-1:250 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CDA02 antibody, anti-eIF 2 alpha antibody, anti-EIF 2 antibody, anti-EIF 2A antibody, anti-EIF-2alpha antibody, anti-EIF2 antibody, anti-EIF2A antibody, anti-EIF2alpha antibody, anti-eIF2S1 antibody, anti-Eukaryotic Translation Initiation Factor 2 alpha antibody, anti-Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody, anti-Eukaryotic translation initiation factor 2 subunit 1 antibody, anti-Eukaryotic translation initiation factor 2 subunit alpha antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser51 |
| General References: | 1. Ernst, H., et al. Cloning and sequencing of complementary DNAs encoding the alpha-subunit of translational initiation factor eIF-2. Characterization of the protein and its messenger RNA. J Biol Chem. 262: 1206-12 (1987). 2. Kimball, S.R. Eukaryotic initiation factor eIF2. Int. J. Biochem. Cell Biol. 31: 25-29 (1999). 3. De Haro, C., et al. The eIF-2alpha kinases and the control of protein synthesis. FASEB J. 10: 1378-1387 (1996). |
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