| CatalogCode: | NB110-56939 |
| ProductName: | eEF2 Antibody |
| Product Description: | Rabbit Monoclonal anti-eEF2 (EP723Y) |
| Clone: | EP723Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen. |
| CrossReactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000 IHC: 1:50-100 ICC: 1:50 |
| Background: | The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-EF2 antibody, anti-Elongation factor 2 antibody, anti-Eukaryotic translation elongation factor 2 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Rapp, G.; Klaudiny, J.; Hagendorff, G.; Luck, M. R.; Scheit, K. H. : Biol. Chem. Hoppe-Seyler 370: 1071-1075, 1989. 2. Carlberg, U., Nilsson, A. and Nygard, O. (1990) Eur. J. Biochem. 191, 639-645 3. Price, N.T., Redpath, N.T., Severinov, K.V., Campbell, D.G., Russell, J.M. and Proud, C.G. (1991) FEBS Lett. 282, 253-258 4. Ryazanov, A.G., Natapov, P.G., Shestakova, E.A., Severin, F.F. and Spirin, A.S. (1988) Biochimie 70, 619-626 |
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