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| Catalog Code: | NB110-56938 |
| Product Name: | eEF-2K Antibody |
| Product Description: | Rabbit Monoclonal anti-eEF-2K (EP724Y) |
| Clone: | EP724Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the Calmodulin binding region of human eEF2 was used as immunogen. |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1: 50,000 IP: 1:20 |
| Background: | Eukaryotic elongation factor-2 kinase (eEF-2k) previously known as Ca2+/calmodulin-dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor-2 (eEf-2) (1). Phosphorylation of eEF-2 by eEF-2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation (2). The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively by phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK) (3-4). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors (5-6). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Calcium/calmodulin dependent eukaryotic elongation factor 2 antibody, anti-Calmodulin dependent protein kinase III antibody, anti-EC 2.7.11.20 antibody, anti-eEF 2 kinase antibody, anti-eEF 2K antibody, anti-EEF2K protein antibody, anti-Elongation factor 2 kinase antibody, anti-Eukaryotic elongation factor 2 kinase antibody, anti-kinase eEF2K antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Ryazanov et al. Proc Nat Acad Sci 94: 4884-4889, 1997 2. Ryazanov AG, et al. Nature 334:170-173, 1988. 3. Diggle, TA, et al. FEBS Lett 457:189-192, 1999. 4. Wang, XW, EMBO J 20:4370-4379, 2001. 5. End D, et al. J Biol Chem 257:9223-9225, 1988. 6. Severinov, KV, et al. New Biol 2: 887-893, 1990. |
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