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| Catalog Code: | NB110-56935 |
| Product Name: | PRKDC/DNA-PK Antibody |
| Product Description: | Rabbit Monoclonal anti-PRKDC/DNA-PK (Y393) |
| Clone: | Y393 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the C-term of human DNA-PK was used as immunogen. This antibody may detect splice isoform 2 |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1: 1,000 IHC: 1:50 ICC: 1:250-500 |
| Background: | DNA-activated serine/threonine protein kinase (DNA-PK) is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required for double- strand brake repair and V(D)J recombination. DNA-PK is composed of a large catalytic subunit of ~470 kDa (DNA-PKcs) and a DNA binding heterodimer of approximately 70- and 80-kDa subunits called Ku. (1-2). Autophosphorylation of DNA-PK correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK phosphorylates Hsp90, p53 and c-Jun (3-4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-DNA dependent protein kinase catalytic subunit antibody, anti-DNA PK catalytic subunit antibody, anti-DNAPK antibody, anti-DNAPK catalytic subunit antibody, anti-DNPK 1 antibody, anti-DNPK1 antibody, anti-HYRC 1 antibody, anti-HYRC antibody, anti-HYRC1 antibody, anti-p350 antibody, anti-p460 antibody, anti-PKRDC antibody, anti-PRKDC antibody, anti-Protein Kinase DNA Activated Catalytic Polypeptide antibody, anti-XRCC 7 antibody, anti-XRCC7 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Anderson, C. W., and Lees-Miller, S. P. (1992) Crit. Rev. Eukaryotic Gene Expression 2, 283-314 2. Anderson, C. W., and Carter, T. H. (1996) Curr. Topics Microbiol. Immunol. 217, 91-111 3. Lees-Miller, S.P., Sakaguchi, K., Ullrich, S.J., Appella, E. and Anderson, C.W. (1992) Mol. Cell. Biol. 12, 5041-5049 4. Bannister, A.J., Gottlieb, T.M., Kouzarides, T. and Jackson, S.P. (1993) Nucleic Acids Res. 21, 1289-1295 |
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