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| Catalog Code: | NB110-56931 |
| Product Name: | Desmin Antibody |
| Product Description: | Rabbit Monoclonal anti-Desmin (Y66) |
| Clone: | Y66 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to C-terminus of human Desmin was used as immunogen. Predicted to cross-react with bovine and pig based on sequence homology. |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500 IHC: 1:500 |
| Background: | Desmin are class-III intermediate filaments found in muscle cells, and are expressed during the formation of muscle cells or fibers. Cytoskeletal intermediate filaments (IFs) are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice (1,2). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. Desmin mutations have been linked to familial cardiac and skeletal myopathy (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Stewart, M. Intermediate filament structure and assembly. Curr. Opin. Cell Biol. 5: 3-11 (1993). 2. Li, Z.L., et al. Human desmin-coding gene: complete nucleotide sequence, characterization and regulation of expression during myogenesis and development. Gene 78: 243-54 (1989). 3. Li, Z.L. and D. Paulin. High level desmin expression depends on a muscle-specific enhancer. J Biol Chem. 266: 6562-70 (1991). 4. Goldfarb, L.G., et al. Missense mutations in desmin associated with familial cardiac and skeletal myopathy. Nat Genet. 19: 402-3 (1998). |
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