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| Catalog Code: | NB110-56924 |
| Product Name: | Beta-catenin Antibody |
| Product Description: | Rabbit Monoclonal anti-Beta-catenin (E247) |
| Clone: | E247 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | A synthetic peptide corresponding to residues near N terminus of human beta catenin. |
| Specificity: | A synthetic peptide corresponding to residues near N-terminus of human â-catenin was used as immunogen. |
| Cross Reactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:5,000-1:10,000 IHC: 1:500 ICC: 1:250 IP: 1:100 |
| Background: | â-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation (1). â-catenin is phosphorylated at four serine and threonine residues at the N-terminus (2). Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33 (2). Wnt signaling antagonizes the action of GSK-3, which in subsequent phosphorylation steps, leads to its degradation (3). Mutations in the â-catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry 1:500 , immunoprecipitation 1:100, Western Blot 1:5,000-1:10,000 , Immunocytochemistry 1:250, |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-Beta catenin antibody, anti-Cadherin associated protein antibody, anti-Catenin beta 1 antibody, anti-CATNB antibody, anti-CTNNB antibody, anti-CTNNB1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Kikuchi, A. Regulation of beta-catenin signaling in the Wnt pathway. Biochem Biophys Res Commun. 268: 243-8 (2000). 2. Hagen, T., and A. Vidal-Puig. Characterisation of the phosphorylation of beta-catenin at the GSK-3 priming site Ser45. Biochem Biophys Res Commun. 294: 324-8 (2002). 3. van Noort, M., et al. Wnt signaling controls the phosphorylation status of beta-catenin. J Biol Chem. 277: 17901-5 (2002). 4. Blaker, H., et al. Beta-catenin accumulation and mutation of the CTNNB1 gene in hepatoblastoma. Genes Chromosomes Cancer. 25: 399-402 (1999). |
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