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| Catalog Code: | NB110-56920 |
| Product Name: | Cytokeratin 8 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-Cytokeratin 8 (pS73) (E431-2) [Ser73] |
| Clone: | E431-2 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser73 of human Cytokeratin 8 was used as immunogen. The antibody only detects Keratin 8 phosphorylated on Serine 73. |
| Cross Reactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000 IHC: 1:500 (antigen retrieval required) ICC: 1:250 IP: 1:100 |
| Background: | Keratin polypeptides 8 and 18 (K8/18) are intermediate filament phosphoglycoproteins that are expressed preferentially in glandular epithelia. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (1-3). Together with actin microfilaments and microtubules, keratin filaments make up the cytoskeletons of vertebrate epithelial cells, forming alpha-helical coiled-coil dimers which associate laterally and end-to-end to form 10-nm diameter filaments (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-card2 antibody, anti-Cardiac autoantigen 2 120kD antibody, anti-CK 8 antibody, anti-ck8 antibody, anti-Cyk 8 antibody, anti-cyk8 antibody, anti-Cytokeratin 8 antibody, anti-Cytokeratin8 antibody, anti-k0 antibody, anti-k2c8 antibody, anti-k8 antibody, anti-Keratin 8 antibody, anti-Keratin type ii cytoskeletal 8 antibody, anti-Keratin8 antibody, anti-KO antibody, anti-Krt 8 antibody, anti-krt8 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser73 |
| General References: | 1. Ku, N.O. and M.B. Omary. Identification and mutational analysis of the glycosylation sites of human keratin 18. J Biol Chem. 270: 11820-7 (1995). 2. Kulesh, D.A. and R.G. Oshima. Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Mol Cell Biol. 8: 1540-50 (1988). 3. Marceau, N. and A. Loranger. Cytokeratin expression, fibrillar organization and subtle function in liver cells. Biochem. Cell Biol. 73: 619-625 (1995). 4. Fuchs, E. Keratins and the skin. Annu. Rev. Cell. Dev. Biol. 11: 123-153 (1995). |
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