| request information: |
|
| Catalog Code: | NB110-56916 |
| Product Name: | Cytokeratin 5 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-Cytokeratin 5 (C-term) (EP1601Y) |
| Clone: | EP1601Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) |
| Specificity: | A synthetic peptide corresponding to residues on the C-terminus of human CK-5 was used as an immunogen. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:10,000 IHC: 1:100 - 250 ICC: 1:100 - 250 |
| Background: | Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Eckert RL, et al. DNA 7(5):337-45, 1988. 2. Trask DK, et al. Proc Natl Acad Sci 87(6)2319-23, 1990 3. Betz RC, et al. Am J Hum Genet 78(3):510-9, 2006. |
order or
more information: | company product webpage |