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| Catalog Code: | NB110-56907 |
| Product Name: | Cytokeratin 14 Antibody |
| Product Description: | Rabbit Monoclonal anti-Cytokeratin 14 (EP1612Y) |
| Clone: | EP1612Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human Cytokeratin 14 was used as an immunogen. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000 IHC: 1:100 - 250 FC: 1:30 IP: 1:50 |
| Background: | Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Cytokeratin 14 (CK-14) is a 50-kDa keratin expressed in abundance in human epidermal cells (1). During anagen, cell proliferation in the germinative matrix of the hair follicle gives rise to the fiber and inner root sheath. The transition from anagen to telogen is marked by downregulation of hair cortical specific keratins and the appearance of CK-14 in the epithelial sac to which the telogen hair fiber is anchored (2). A family with recessive epidermolysis bullosa simplex lacked expression of the basal cell keratin 14. The patients had severe generalized skin blistering that improved slightly with age. The basal cells of the patients did not express keratin 14 and contained no keratin intermediate filaments. The expression of keratin 5, the obligate copolymer of keratin 14, was slightly reduced (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-CK 14 antibody, anti-ck14 antibody, anti-Cytokeratin 14 antibody, anti-Cytokeratin14 antibody, anti-Dowling Meara antibody, anti-ebs3 antibody, anti-ebs4 antibody, anti-Epidermolysis bullosa simplex antibody, anti-k14 antibody, anti-Keratin 14 antibody, anti-Keratin type I cytoskeletal 14 antibody, anti-Keratin14 antibody, anti-Koebner antibody, anti-krt14 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Marchuk D, et al. Proc Natl Acad Sci 82(6):1609-13, 1985 2. Bowden PE, et al. J Invest Dermatol 110(2):158-64, 1998 3. Jonkman MF, et al. J Invest Dermatol 107(5):764-9, 1996 |
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