| CatalogCode: | NB110-56900 |
| ProductName: | Cyclin B1 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-Cyclin B1 (C-term) (Y106) |
| Clone: | Y106 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues in the C-terminus of Human cyclin B1 |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members. |
| CrossReactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml protein A purified Rabbit ascites. |
| Uses: | WB: 1:5,000-20,000 IHC: 1:100 ICC: 1:50 Flow Cytometry: 1:100 IP: 1:100 |
| Background: | Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1-Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | anti-CCNB 1 antibody, anti-CCNB antibody, anti-CCNB1 antibody, anti-G2 mitotic specific cyclin B1 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Morgan, D.O..1997. Cyclin-dependent kinases: engines, clocks, and microprocessors. Annu. Rev. Cell Dev. Biol. 13: 261-291 2. Dunphy, W.G.. 1994. The decision to enter mitosis. Trends Cell Biol. 4: 202-207 3. Gallant, P., and E.A. Nigg. 1992. Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells. J. Cell Biol. 117: 213-224 |
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