| CatalogCode: | NB110-56897 |
| ProductName: | Phospho CrkL (pY207) Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho CrkL (pY207) (EP270Y) [Tyr207] |
| Clone: | EP270Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human CrkL was used as immunogen. The antibody only detects CrkL phosphorylated on Tyrosine 207. |
| CrossReactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:50,000 IP: 1:50 |
| Background: | CrkL (Crk-like), a 36kDa protein predominantly expressed in hematopoietic cell lines, is a member of the Crk adapter protein family. Lacking any intrinsic enzymatic activity, CrkL is composed of one SH2 domain and two SH3 domains (1). The SH2 domain of CrkL as been shown to interact with c-Cbl, Cas, Hef-1 and Paxillin (2); while the N-terminal SH3 can bind to c-ABL, Sos1 and C3G (3). Additionally, the phosphorylated form of CrkL (pY207) binds to BCr-ABL oncogene in chronic myelogenous leukemia cells (4). Specificity: |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu, Mu, Rt(-) |
| ALTnames: | anti-Crk L antibody, anti-Crk like protein antibody, anti-Crkol antibody, anti-HGNC:2363 antibody, anti-Oncogene CrkL antibody, anti-V crk avian sarcoma virus CT10 oncogene homolog like antibody, anti-V crk sarcoma virus CT10 oncogene homolog avian like antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Hoeve J, et al. Oncogene 8:2469-2474, 1993. 2. Kyono, W, et al. J. Immunol. 161:5555-5563, 1998. 3. Sattler, M, et al. J. Biol. Chem. 272:14320-14326, 1997. 4. Ling, P, et al. Mol. Cell. Biol. 19:1359-1368, 1999. |
| Phosphorylated: | Tyr207 |
|