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| Catalog Code: | NB110-56895 |
| Product Name: | CrkL Antibody |
| Product Description: | Rabbit Monoclonal anti-CrkL (Y244) |
| Clone: | Y244 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the second SH3 domain and near C-terminus of human Crk-like protein was used as immunogen. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:500 IHC: 1:50 ICC: 1:50 IP: 1:10 |
| Background: | CrkL (Crk-like), a 36kDa protein predominantly expressed in hematopoietic cell lines, is a member of the Crk adapter protein family. Lacking any intrinsic enzymatic activity, CrkL is composed of one SH2 domain and two SH3 domains (1). The SH2 domain of CrkL as been shown to interact with c-Cbl, Cas, Hef-1 and Paxillin (2); while the N-terminal SH3 can bind to c-ABL, Sos1 and C3G (3). Additionally, the phosphorylated form of CrkL (pY207) binds to BCr-ABL oncogene in chronic myelogenous leukemia cells (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-Crk L antibody, anti-Crk like protein antibody, anti-Crkol antibody, anti-HGNC:2363 antibody, anti-Oncogene CrkL antibody, anti-V crk avian sarcoma virus CT10 oncogeneomolog like antibody, anti-V crk sarcoma virus CT10 oncogene homolog avian like antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. ten Hoeve J., Morris C., Heisterkamp N., Groffen J.; Oncogene 8:2469-2474(1993). 2. Kyono, W. T., de Jong, R., Park, R. K., Liu, Y., Heisterkamp, N., Groffen, J., and Durden, D. L. (1998) J. Immunol. 161, 5555-5563 3. Sattler, M., Salgia, R., Shrikhande, G., Verma, S., Uemura, N., Law, S. F., Golemis, E. A., and Griffin, J. D. (1997) J. Biol. Chem. 272, 14320-14326 4. Ling, P., Yao, Z., Meyer, C. F., Wang, X. S., Oehrl, W., Feller, S. M., and Tan, T. H. (1999) Mol. Cell. Biol. 19, 1359-1368 |
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