| CatalogCode: | NB110-55718 |
| ProductName: | Chk1 (N-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-Chk1 (N-term) (E250) |
| Clone: | E250 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to N-terminal residues of human Chk1 was used as immunogen. |
| CrossReactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000ICC: 1:50Flow Cytometry: n/dIP: n/d |
| Background: | Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt(-) |
| ALTnames: | anti-CHEK 1 antibody, anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe) antibody, anti-Cell cycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Chk1 antibody, anti-Serine/threonine protein kinase Chk1 antibody, anti-CHEK 1 antibody, anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe) antibody, anti-Cell cycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Serine/threonine protein kinase Chk1 antibod antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Martinho, R.G., et al. Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. EMBO J. 17: 7239-7249 (1998). 2. Zhao, H. and H. Piwnica-Worms. ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1. Mol. Cell. Biol. 21: 4129-4139 (2001). 3. Zeng, Y., et al. Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1. Nature 395: 507-510 (1998). 4. Sanchez, Y., et al. Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277: 1497-501 (1997). |
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