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| Catalog Code: | NB110-55717 |
| Product Name: | Chk1 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-Chk1 (C-term) (EP691Y) |
| Clone: | EP691Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000IHC: 1:250 - 500ICC: 1:100 - 250FC: 1:160 |
| Background: | Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-CHEK 1 antibody, anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe) antibody, anti-Cell cycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Chk1 antibody, anti-Serine/threonine protein kinase Chk1 antibody, anti-CHEK 1 antibody, anti-CHEK1 antibody, anti-CHK1 checkpoint homolog (S. pombe) antibody, anti-Cell cycle checkpoint kinase antibody, anti-Chk 1 antibody, anti-Serine/threonine protein kinase Chk1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Martinho RG, et al. EMBO J. 17:7239-7249, 1998. 2. Zhao H, et al. Mol Cell Biol 21:4129-4139, 2001. 3. Zeng Y, et al. Nature 395:507-510, 1998. 4. Sanchez Y, et al. Science 277:1497-1501, 1997. |
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