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| Catalog Code: | NB110-55714 |
| Product Name: | Cdk5 (C-term) protein Antibody |
| Product Description: | Rabbit Monoclonal anti-Cdk5 (C-term) protein (EP715Y) |
| Clone: | EP715Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the C-terminus of human Cdk5 was used as immunogen. |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2,000-10,000IHC: 1:100-250ICC: 1:100-250Flow Cytometry: 1:30IP: 1:50 |
| Background: | Cyclin-dependent protein kinase 5 (Cdk5) is a unique member of the family of cyclin-dependent kinases that is not directly involved in the progression of proliferating cells through the cell cycle (1). Distributed in most tissues and various cell lines, Cdk5 is active primarily in neuronal tissues, because of its association with neuron-specific activators such as p35 (a neural-specific regulatory subunit of Cdk5) (2). In addition to its role in muscle and neuronal differentiation, Cdk5 also contributes to the assembly, organization and stability of the axonal cytoskeleton in neurons, promotes neurite outgrowth and axon guidance in developing neurons, participates in the regulation of synaptic transmission and provides an additional pathway to cellular apoptosis (3-5). Cdk5 phosphorylates MAP1b, Tau, neurofilament proteins, caldesmon and proteins of the synaptic vesicles. Deregulation of Cdk5 has profound cytotoxic effects and is implicated in the development of neurodegenerative diseases such as Alzheimer's disease and amyotrophic lateral sclerosis (6). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Cdk 5 antibody, anti-Cell division protein kinase 5 antibody, anti-Crk6 antibody, anti-PSSALRE antibody, anti-Serine threonine protein kinase PSSALRE antibody, anti-TPKII catalytic subunit antibody, anti-Tau protein kinase II catalytic subunit antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Tsai, L.H., Takahashi, T., Caviness, V. Jr & Harlow, E. (1993) Development 119, 1029-1040. 2. J Tsai, L.H., Delalle, I., Caviness, V.S. Jr, Chae, T. & Harlow, E. (1994) Nature 371, 419-423 3. Miyasaka, H., Okabe, S., Ishiguro, K., Uchida, T. & Hirokawa, N. (1993) J. Biol. Chem. 268, 22695-22702 4. Rosales, J.L., Nodwell, M.J., Johnston, R.N. & Lee, K.Y. (2000) J. Cell Biochem. 78, 151-159 5. Zhang, Q., Ahuja, H.S., Zakeri, Z.F. & Wolgemuth, D.J. (1997) Dev Biol. 183, 222-233. 6. Patrick et al. Nature 402: 615-622, 1999. |
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