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| Catalog Code: | NB110-55713 |
| Product Name: | Cdk2 (C-term) Antibody |
| Product Description: | Rabbit Monoclonal anti-Cdk2 (C-term) (E304) |
| Clone: | E304 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human Cdk2 was used as immunogen. |
| Cross Reactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:5,000ICC: 1:10015-IP: 1:40 |
| Background: | Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kDa nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-CDK2 antibody, anti-Cdc2 related protein kinase antibody, anti-Cdk 2 antibody, anti-Cell division kinase 2 antibody, anti-Cell division protein kinase 2 antibody, anti-Cyclin dependent kinase 2 antibody, anti-p33 protein kinase antibody, anti-p33(CDK2) antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Morgan, D.O. Principles of CDK regulation. Nature 374: 131-134 (1995). 2. Elledge, S.J., et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907-2911 (1992). 3. Gu, Y., et al. Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15. EMBO J. 11: 3995-4005 (1992). 4. Fesquet, D., et al. The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues. EMBO J. 12: 3111-3121 (1993). |
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