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| Catalog Code: | NB110-55707 |
| Product Name: | Cdc2 Antibody |
| Product Description: | Rabbit Monoclonal anti-Cdc2 (E53) |
| Clone: | E53 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues of human Cdc2 was used as immunogen. Predicted to cross-react with most species, based on sequence homology. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:5,000IP: 1:40 |
| Background: | During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the Cdc25C (Cat# 1302-1/ 1490-1) phosphatase (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-CDK 1 antibody, anti-CDK1 antibody, anti-Cdc 2 antibody, anti-Cell Divsion Cycle 2 Protein antibody, anti-Cell division control protein 2 antibody, anti-Cell division control protein 2 homolog antibody, anti-Cell division cycle 2 G1 to S and G2 to M antibody, anti-Cyclin Dependent Kinase 1 antibody, anti-DKFZp686L20222 antibody, anti-MGC111195 antibody, anti-p34 Protein Kinase antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Norbury, C. and P. Nurse. Animal cell cycles and their control. Annu. Rev. Biochem. 61: 441-470 (1992). 2. Atherton-Fessler, S. et al. Mechanisms of p34cdc2 regulation. Mol. Cell. Biol. 13: 1675-1685 (1993). 3. Watanabe, N. et al. Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle. EMBO J. 14: 1878-1891 (1995). 4. Galaktionov, K. et al. Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes Dev. 9: 1046-1058 (1995). |
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