| request information: |
|
| Catalog Code: | NB110-55697 |
| Product Name: | CD3 epsilon Antibody |
| Product Description: | Rabbit Monoclonal anti-CD3 epsilon (E272) |
| Clone: | E272 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in C-terminal cytoplasmic domain of human CD-3 epsilon chain was used as immunogen. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | IHC: 1:100ICC: 1:100-250IP: 1:50 |
| Background: | CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR) (1). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C (1,2). CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-CD 3E antibody, anti-CD3 TCR complex antibody, anti-CD3E antibody, anti-CD3e molecule epsilon antibody, anti-T cell surface antigen T3/Leu 4 epsilon chain antibody, anti-T cell surface antigen T3/Leu4 epsilon antibody, anti-T cell surface glycoprotein CD3 epsilon chain precursor antibody, anti-T3E antibody, anti-TCRE antibody, anti-TiT3 complex antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Weiss, A., et al. Signal transduction by the T cell antigen receptor. Sem. Immunol. 3: 313-324 (1991). 2. Siegel, J.N., et al. Multiple signal transduction pathways activated through the T cell receptor for antigen. Semin Immunol. 3: 325-34 (1991). 3. Futterer, K., et al. Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide. J Mol Biol. 281: 523-37 (1998). |
order or
more information: | company product webpage |
|