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| Catalog Code: | NB110-55673 |
| Product Name: | Cbl Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-Cbl (pY774) (E160) [Tyr774] |
| Clone: | E160 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyr774 of human Cbl was used as immunogen. The antibody detects Cbl phosphorylated on Tyrosine 774. |
| Cross Reactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000IHC: 1:25ICC: 1:25Flow Cytometry: 1:20IP: 1:20 |
| Background: | c-Cbl, product of c-cbl proto-oncogene, is a 120 kDa cytoplasmic protein involved in tyrosine kinase-dependent signaling pathways (1-3). c-Cbl is one of the earliest targets of tyrosine phosphorylation in response to a number of cellular stimuli. For instance, stimulation of growth factor receptors by EGF, PDGF, NGF and FGF, results in c-Cbl tyrosine phosphorylation, in lymphoid and other cell lines (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-C CBL antibody, anti-Cas-Br-M (murine) ecotropic retroviral transforming sequence antibody, anti-Casitas B lineage lymphoma proto oncogene antibody, anti-CBL 2 antibody, anti-CBL2 antibody, anti-E3 ubiquitin protein ligase CBL antibody, anti-Oncogene CBL2 antibody, anti-Proto oncogene c CBL antibody, anti-RING finger protein 55 antibody, anti-RNF55 antibody, anti-Signal transduction protein CBL antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr774 |
| General References: | 1. Langdon, W. Y., et al. v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas. Proc. Natl. Acad. Sci. USA 86: 1168-72 (1989). 2. Blake, T. J., et al. The truncation that generated the v-cbl oncogene reveals an ability for nuclear transport, DNA binding and acute transformation. EMBO J. 12: 2017-26 (1993). 3. Joazeiro, C. A., et al. The tyrosine kinase negative regulator c-Cbl as a RING-type, E2-dependent ubiquitin-protein ligase. Science 286: 309-12 (1999). 4. Galisteo, M. L., et al. Tyrosine phosphorylation of the c-cbl proto-oncogene protein product and association with epidermal growth factor (EGF) receptor upon EGF stimulation. J. Biol. Chem. 270: 20242-5 (1995). |
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