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| Catalog Code: | NB110-55669 |
| Product Name: | Caspase-9 (pro/p35) Antibody |
| Product Description: | Rabbit Monoclonal anti-Caspase-9 (pro/p35) (E23) |
| Clone: | E23 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to C-terminus of human Caspase-9 p35 subunit was used as immunogen. The antibody should recognize both pro-form (46kDa) and p35 cleaved-form of Caspase-9. |
| Cross Reactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000IHC: 1:50-100ICC: 1:50Flow Cytometry: 1:100IP: 1:100 |
| Background: | Caspase-9 (ICE-LAP6, Mch-6, Apaf-3) is a member of the cysteine aspartic acid protease, or caspase, family. Pro-Caspase-9 exists as a 46 kDa isomer that is cleaved by apoptotic stimulation, and functions as the major initiator caspase in programmed cell death, or apoptosis (1-4). Upon stimulation, cytochrome c is released from mitochondria, and associates with the Pro-Caspase-9/Apaf 1 complex. This complex in turn activates Pro-Caspase-9 by cleaving it into an active 35 kDa fragment and a small 10 kDa fragment (1,2). Activated Caspase-9 cleaves Caspase-3 as well as poly (ADP-ribose) polymerase (PARP) to initiate a caspase cascade and apoptosis (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-APAF 3 antibody, anti-APAF3 antibody, anti-Apoptosis related cysteine peptidase antibody, anti-Apoptotic protease activating factor 3 antibody, anti-Apoptotic protease Mch 6 antibody, anti-CASP 9 antibody, anti-CASP9 antibody, anti-Caspase 9c antibody, anti-Caspase9 antibody, anti-ICE LAP6 antibody, anti-ICE like apoptotic protease 6 antibody, anti-MCH6 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Kuida, K. et al. Reduced Apoptosis and Cytochrome c-Mediated Caspase Activation in Mice Lacking Caspase 9. Cell 94: 325-337 (1998). 2. Li, P. et al. Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade. Cell 91: 479-489 (1997). 3. Duan, H. et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271: 16720-24 (1996.). 4. Srinivasula, S.M. et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 271: 27099-27106 (1996). |
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