| CatalogCode: | NB110-55668 |
| ProductName: | Pro-Caspase-9 Antibody |
| Product Description: | Rabbit Monoclonal anti-Pro-Caspase-9 (E84) |
| Clone: | E84 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues near N-terminus of human Caspase-9 was used as immunogen. This antibody only recognises the Pro form of Caspase-9 (46kDa). |
| CrossReactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:2,000IHC: 1:25Flow Cytometry: 1:50 |
| Background: | 1. Muzio, M. et al. FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signaling complex. Cell 85: 817-827 (1996). 2. Boldin, M.P. et al. Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1 and TNF receptor-induced cell death. Cell 85: 803-815 (1996). 3. Srinivasula, S.M. et al. Molecular ordering of the Fas-apoptotic pathway: the Fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteine proteases. Proc. Natl. Acad. Sci. U S A 93(25): 14486-91 (1996). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| NovusRef: | 1. Nikiforov MA, Riblett M, Tang W-H, et al. Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition. PNAS. 2007 December 4, 2007;104(49):19488-93. |
| AppSummary: | FACS, IHC, WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-APAF3 antibody, anti-Apoptotic Protease Activating Factor 3 antibody, anti-Apoptotic Protease Mch 6 antibody, anti-CASP9 antibody, anti-Caspase 9 Precursor antibody, anti-Caspase 9 Subunit p10 antibody, anti-Caspase 9 Subunit p35 antibody, anti-ICE Like Apoptotic Protease 6 antibody, anti-ICELAP6 antibody, anti-MCH6 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Kuida, K. et al. Reduced Apoptosis and Cytochrome c-Mediated Caspase Activation in Mice Lacking Caspase 9. Cell 94: 325-337 (1998). 2. Li, P. et al. Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade. Cell 91: 479-489 (1997). 3. Duan, H. et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 271: 16720-24 (1996.). 4. Srinivasula, S.M. et al. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 271: 27099-27106 (1996). |
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