| CatalogCode: | NB110-55665 |
| ProductName: | Caspase-7 Antibody |
| Product Description: | Rabbit Monoclonal anti-Caspase-7 (Y33) |
| Clone: | Y33 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-7 was used as immunogen. The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7. |
| CrossReactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000IHC: 1:50ICC: 1:50 |
| Background: | Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1, Mch3, ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1,2,3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation, Caspase-7 is translocated to the mitochondria, and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2,3) and endoplasmic reticular-specific substrate, sterol regulatory element-binding protein 1 (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | anti-apoptosis-related cysteine peptidase antibody, anti-Apoptotic protease Mch-3 antibody, anti-CASP7 antibody, anti-CMH-1 antibody, anti-ICE-LAP3 antibody, anti-ICE-like apoptotic protease 3 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Duan, H., et al. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. J. Biol. Chem. 271: 1621-5 (1996). 2. Lippke, J.A., et al. Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32. J. Biol. Chem. 271: 1825-8 (1996). 3. Fernandes-Alnemri, T., et al. Mch3, a novel human apoptotic cysteine protease highly related to CPP32. Cancer Res. 55: 6045-52 (1995). 4. Chandler, J.M., et al. Different Subcellular Distribution of Caspase-3 and Caspase-7 following Fas-induced Apoptosis in Mouse Liver. J. Biol. Chem. 273: 10815-10818 (1998). |
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