| CatalogCode: | NB110-55664 |
| ProductName: | Caspase-7 Antibody |
| Product Description: | Rabbit Monoclonal anti-Caspase-7 (E22) |
| Clone: | E22 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues near N-terminus of human Caspase-7 was used as immunogen. The antibody should recognize both pro-form and p20 cleaved-form. The antibody does not cross-react with other Caspase family members. |
| CrossReactivity: | Reacts in Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000IHC: 1:50-1:100ICC: 1:100IP: 1:10 |
| Background: | Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-7 (CMH-1, Mch3, ICE-LAP3) is an effector caspase involved in the activation cascade for apoptosis execution (1,2,3). Pro-Caspase-7 is a 35-kDa protein localized to the cytoplasm and expressed in a variety of tissues and cell lines (1). Upon activation, Caspase-7 is translocated to the mitochondria, and proceeds to proteolytically cleave poly(ADP-ribose) polymerase (PARP) (2,3) and endoplasmic reticular-specific substrate, sterol regulatory element-binding protein 1 (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| NovusRef: | 1. Nikiforov MA, Riblett M, Tang W-H, et al. Tumor cell-selective regulation of NOXA by c-MYC in response to proteasome inhibition. PNAS. 2007 December 4, 2007;104(49):19488-93. |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu(-), Rt(-) |
| ALTnames: | anti-Apoptosis Related Cysteine Protease antibody, anti-Apoptotic Protease MCH3 antibody, anti-CASP 7 antibody, anti-CASP7 antibody, anti-Caspase 7 apoptosis related cysteine peptidase antibody, anti-Caspase 7 apoptosis related cysteine protease antibody, anti-Caspase7 antibody, anti-CMH1 antibody, anti-ICE LAP3 antibody, anti-ICE like Apoptotic Protease 3 antibody, anti-Lice2 alpha/beta/gamma antibody, anti-MCH3 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Duan, H., et al. ICE-LAP3, a novel mammalian homologue of the Caenorhabditis elegans cell death protein Ced-3 is activated during Fas- and tumor necrosis factor-induced apoptosis. J. Biol. Chem. 271: 1621-5 (1996). 2. Lippke, J.A., et al. Identification and characterization of CPP32/Mch2 homolog 1, a novel cysteine protease similar to CPP32. J. Biol. Chem. 271: 1825-8 (1996). 3. Fernandes-Alnemri, T., et al. Mch3, a novel human apoptotic cysteine protease highly related to CPP32. Cancer Res. 55: 6045-52 (1995). 4. Chandler, J.M., et al. Different Subcellular Distribution of Caspase-3 and Caspase-7 following Fas-induced Apoptosis in Mouse Liver. J. Biol. Chem. 273: 10815-10818 (1998). |
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