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| Catalog Code: | NB110-55659 |
| Product Name: | Pro-Caspase-3 Antibody |
| Product Description: | Rabbit Monoclonal anti-Pro-Caspase-3 (E61) |
| Clone: | E61 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to N-terminal residues of human Pro-Caspase-3 was used as immunogen. The antibody only recognizes the pro-form of Caspase-3. It does not react with the cleaved forms (active enzyme) of Caspase-3. |
| Cross Reactivity: | Reacts in Human and Mouse. Does not react in Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:1,000 IHC: 1:500 (with antigen retrieval) ICC: 1:100 Flow Cytometry: 1:50 IP: 1:10 |
| Background: | Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt(-), |
| Alternate Names: | anti-Apoptosis related cysteine peptidase antibody, anti-CASP 3 antibody, anti-CASP3 antibody, anti-CPP 32 antibody, anti-CPP32 antibody, anti-CPP32B antibody, anti-Caspase 3 antibody, anti-Caspase 3 apoptosis related cysteine peptidase antibody, anti-Caspase3 antibody, anti-PARP cleavage protease antibody, anti-Procaspase 3 antibody, anti-Procaspase3 antibody, anti-SCA 1 antibody, anti-SCA1 antibody, anti-SREBP cleavage activity 1 antibody, anti-Yama antibody, anti-Yama protein antibody, anti-caspase 3 preproprotein antibody, anti-cysteine protease CPP32 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Fernandes-Alnemri, T. et al. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269:30761-4 (1994). 2. Tewari, M. et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-9 (1995). 3. Nicholson, D.W. et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37-43 (1995). |
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