| request information: |
|
| Catalog Code: | NB110-55657 |
| Product Name: | Pro-Caspase-3 Antibody |
| Product Description: | Rabbit Monoclonal anti-Pro-Caspase-3 (E87) |
| Clone: | E87 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues from within the first 100 amino acids in the p17 subunit of Human Capase 3. |
| Specificity: | A synthetic peptide corresponding to residues in p17 subunit of human Caspase-3 was used as immunogen. The antibody should recognize both pro-form (35kDa) and p17 cleaved-form of Caspase-3. |
| Cross Reactivity: | Reacts in Human, and Rat. Does not react in Mouse. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:5,000 IHC: 1:25-50 ICC: 1:25 IP: 1:10 |
| Background: | Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry , immunoprecipitation , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt, |
| Alternate Names: | anti-Apopain antibody, anti-Apopain precursor antibody, anti-CASP 3 antibody, anti-CASP3 antibody, anti-CPP 32 antibody, anti-CPP32 antibody, anti-CPP32B antibody, anti-Caspase 3 apoptosis related cysteine protease antibody, anti-Caspase 3 p12 subunit antibody, anti-Caspase 3 p17 subunit antibody, anti-Caspase3 antibody, anti-Cysteine protease CPP32 antibody, anti-EC 3.4.22.56 antibody, anti-Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody, anti-ICE3 antibody, anti-LICE antibody, anti-PARP cleavage protease antibody, anti-SCA 1 antibody, anti-SCA1 antibody, anti-SREBP cleavage activity 1 antibody, anti-Yama protein antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Fernandes-Alnemri, T. et al. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J. Biol. Chem. 269:30761-4 (1994). 2. Tewari, M. et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81: 801-9 (1995). 3. Nicholson, D.W. et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376: 37-43 (1995). |
order or
more information: | company product webpage |
|