| request information: |
|
| Catalog Code: | NB110-55656 |
| Product Name: | Caspase-2 Antibody |
| Product Description: | Rabbit Monoclonal anti-Caspase-2 (Y154) |
| Clone: | Y154 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to residues in the C terminus of Human Caspase 2 p18 subunit. |
| Specificity: | A synthetic peptide corresponding to C-terminus of human Caspase-2 p18 subunit was used as immunogen. The antibody should recognize both pro-form (~50kDa) and p18 cleaved-form of Caspase-2. Predicted to detect the splice isoform (35 kDa) based on sequence homology. |
| Cross Reactivity: | Reacts in Human. does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Background: | Caspase-2 (Nedd2/ICH-1) is part of a family of aspartate-specific cysteine proteases that participate in programmed cell death, or apoptosis. Caspase-2 is localized to the nucleus, and has been shown to play roles in both positive and negative regulation of apoptosis (1). Upon apoptotic stimulation, pro-caspase-9 is cleaved in a series of steps to produce an 18-kDa large subunit and a 12-kDa small fragment (2). Caspase-2 can be a nuclear initiator caspase, as it has been shown to precede the processing and activation of caspase-8, -9, and -3 (3). Caspase-2 might also act as an effector caspase; in apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of caspase-9 and caspase-3 activation (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunoprecipitation 1:20, Western Blot 1:500 |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-Apoptosis related cysteine peptidase antibody, anti-CASP 2 antibody, anti-CASP2 antibody, anti-Casp 2L Pro antibody, anti-Caspase 2 apoptosis related cysteine protease antibody, anti-Caspase2 antibody, anti-Developmentally down regulated 2 antibody, anti-ICH 1 antibody, anti-ICH 1 protease antibody, anti-ICH 1L antibody, anti-ICH 1L/1S antibody, anti-ICH1 antibody, anti-ICH1 protease antibody, anti-ICH1L antibody, anti-ICH1L/1S antibody, anti-NEDD 2 antibody, anti-NEDD2 antibody, anti-NEDD2 apoptosis regulatory gene antibody, anti-Neural precursor cell expressed antibody, anti-Neural precursor cell expressed developmentally down regulated 2 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Wang, L., et al. Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. Cell 78: 739-50 (1994). 2. Li, H., et al. Activation of caspase-2 in apoptosis. J. Biol. Chem 272: 21010-21017 (1997). 3. Dirsch, V.M., et al. Apoptosis signaling triggered by the marine alkaloid ascididemin is routed via caspase-2 and JNK to mitochondria. Oncogene 23: 1586-1593 (2004). 4. Paroni, G., et al. Caspase-2-induced apoptosis is dependent on caspase-9, but its processing during UV- or tumor necrosis factor-dependent cell death requires caspase-3. J. Biol. Chem. 276: 21907-21915 (2001). |
order or
more information: | company product webpage |
|