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| Catalog Code: | NB110-55647 |
| Product Name: | Caldesmon Antibody |
| Product Description: | Rabbit Monoclonal anti-Caldesmon (E89) |
| Clone: | E89 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser789 of human Caldesmon was used as immunogen. . |
| Cross Reactivity: | Reacts in Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:10,000-20,000 IHC: 1:250 ICC: 1:250 Flow Cytometry: 1:20 IP: 1:80 |
| Background: | Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CAD antibody, anti-CALD 1 antibody, anti-CALD1 antibody, anti-CDM antibody, anti-Caldesmon 1 Isoform 1 antibody, anti-Caldesmon 1 Isoform 2 antibody, anti-Caldesmon 1 Isoform 3 antibody, anti-Caldesmon 1 Isoform 4 antibody, anti-Caldesmon 1 Isoform 5 antibody, anti-Caldesmon 1 antibody, anti-Caldesmon1 antibody, anti-H CAD antibody, anti-HCAD antibody, anti-L CAD antibody, anti-LCAD antibody, anti-MGC21352 antibody, anti-NAG22 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Humphrey, M.B., et al. Cloning of cDNAs encoding human caldesmons. Gene 112: 197-204 (1992). 2. Bryan, J., et al. Cloning and expression of a smooth muscle caldesmon. J Biol Chem. 264: 13873-9 (1989). 3. Bryan, J. Caldesmon: fragments, sequence, and domain mapping. Ann N Y Acad Sci. 599: 100-10 (1990). 4. Fraser, I.D., et al. The inhibitory complex of smooth muscle caldesmon with actin and tropomyosin involves three interacting segments of the C-terminal domain 4. Biochemistry 36: 5483-92 (1997). |
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