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| Catalog Code: | NB110-55560 |
| Product Name: | Btk Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Btk Phospho (pY551) (EP267Y) [Tyr551] |
| Clone: | EP267Y |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Tyrosine 551 of human BTK. |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 551 of human Btk was used as immunogen. The antibody will detect Btk phosphorylation on Tyrosine 551. |
| Cross Reactivity: | Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml IgG purified Rabbit ascites. |
| Uses: | WB: 1: 2,000ICC: 1: 100-250 |
| Background: | Btk (Bruton tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. Btk is composed of a pleckstrin domain, an SH3 and SH2 domain, a catalytic kinase domain and a TEC homology domain (1). BTK plays a crucial role in B cell activation and development (2). Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner (3) Btk can be activated through intramolecular autophosphorylation at tyrosine 551 and that Btk autophosphorylation is required for Btk catalytic functions (4). Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA). Patients with this disease are unusually prone to bacterial infection but not to viral infection (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-AGMX 1 antibody, anti-AGMX1 antibody, anti-AT antibody, anti-ATK antibody, anti-Agammaglobulinaemia tyrosine kinase antibody, anti-B cell progenitor kinase antibody, anti-BPK antibody, anti-Bruton agammaglobulinemia tyrosine kinase antibody, anti-Bruton tyrosine kinase antibody, anti-Bruton's Tyrosine Kinase antibody, anti-IMD 1 antibody, anti-IMD1 antibody, anti-MGC126261 antibody, anti-MGC126262 antibody, anti-OTTHUMP00000063593 antibody, anti-PSCTK 1 antibody, anti-PSCTK1 antibody, anti-Tyrosine protein kinase BTK antibody, anti-XLA antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Tyr551 |
| General References: | 1. Tsukada, S., Saffaran, D., Rawlings, D. & Witte, O. (1993) Cell 72, 279-290 2. Rawlings, D. J. & Witte, O. N. (1995) Semin. Immunol. 7, 237-246 3. Vassilev, A., Z. Ozer, C. Navara, S. Mahajan, and F. M. Uckun. 1999. J. Biol. Chem. 274:1646-1656 4. Mahajan S, Fargnoli J, Burkhardt A L, Kut S A, Saouaf S J, Bolen J B. Mol Cell Biol. 1995;15:5304-5311. 5. Yun Qiu, Dan Robinson, Tom G. Pretlow, and Hsing-Jien Kung, Cell Biology, Vol. 95, Issue 7, 3644-3649, March 31, 1998 |
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