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| Catalog Code: | NB110-55557 |
| Product Name: | Bid Antibody |
| Product Description: | Rabbit Monoclonal anti-Bid |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | A synthetic peptide corresponding to residues before the BH3 domain of human Bid. |
| Specificity: | A synthetic peptide corresponding to residues before the BH3 domain of human Bid was used as immunogen. The antibody does not cross-react with other Bcl-2 family members. |
| Cross Reactivity: | Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Background: | Bid, BH3 interacting domain death agonist, is a pro-apoptotic member of the Bcl-2 family of proteins that regulate apotosis. Bid is localized to the cytosol in an inactive precursor form. Upon caspase-8 binding in the Fas signaling pathway, Bid is cleaved and the active Bid fragment translocates to mitochondria and induces cytochrome c release and the resulting apoptotic cascade (1-3). Bid contains a BH3 domain which allows it to dimerize with and counter the death repressor effects of Bcl-2. BID has also been shown to heterodimerize with Bcl-x and the death agonist Bax (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol. |
| Application Summary: | flow cytometry / FACS analysis 1:50, immunohistochemistry 1:100, immunoprecipitation 1:10, Western Blot 1:1,000, Immunocytochemistry 1:100, |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-Apoptotic death agonist BID antibody, anti-Apoptotic death agonist antibody, anti-BH3 interacting domain death agonist antibody, anti-HGNC:1050 antibody, anti-Human BID coding sequence antibody, anti-MGC15319 antibody, anti-MGC42355 antibody, anti-p22 BID antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Gross, A. et al. Caspase Cleaved BID Targets Mitochondria and Is Required for Cytochrome c Release, while BCL-XL Prevents This Release but Not Tumor Necrosis Factor-R1/Fas Death. J. Biol. Chem. 274, 1156-1163 (1999). 2. Li, H. et al. Cleavage of BID by Caspase 8 Mediates the Mitochondrial Damage in the Fas Pathway of Apoptosis. Cell 94, 491-501 (1998). 3. Luo, X. et al. Bid, a Bcl2 Interacting Protein, Mediates Cytochrome c Release from Mitochondria in Response to Activation of Cell Surface Death Receptors. Cell 94, 481-490 (1998). 4. Wang, K. et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 10(22): 2859-69 (1996). |
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