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| Catalog Code: | NB110-55556 |
| Product Name: | Beclin-1 Antibody |
| Product Description: | Rabbit Monoclonal anti-Beclin-1 (EPR1733Y) |
| Clone: | EPR1733Y |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide corresponding to residues near the C-terminus of human Beclin 1. |
| Specificity: | A synthetic peptide corresponding to residues near the C-terminus of human Beclin-1 was used as an immunogen. |
| Cross Reactivity: | Human. Does not react in Mouse or Rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | WB: 1:5000IHC: 1:50ICC: 1:50 - 100IP: 1:50 |
| Background: | The Beclin-1 (BECN1) gene encodes a 60-kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity and tumorigenesis. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry , immunoprecipitation , Western Blot , Immunocytochemistry |
| Species Summary: | Hu, Mu(-), Rt(-), |
| Alternate Names: | anti-ATG 6 antibody, anti-ATG6 antibody, anti-BECN 1 antibody, anti-BECN1 antibody, anti-Beclin1 antibody, anti-Coiled coil myosin like BCL2 interacting protein antibody, anti-GT197 antibody, anti-Protein GT197 antibody, anti-VPS 30 antibody, anti-VPS30 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Aita VM, et al. Genomics 59(1):59-65, 1999 2. Liang XH, et al. Nature 402(6762):672-6, 1999 3. Liang XH, et al. Cancer Res 61(8):3443-9, 2001 |
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