| CatalogCode: | NB110-55551 |
| ProductName: | Bcl-2 Antibody |
| Product Description: | Rabbit Monoclonal anti-Bcl-2 (E17) |
| Clone: | E17 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2. |
| Specificity: | A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2 was used as immunogen. The antibody does not cross-react with other Bcl-2 family members. |
| CrossReactivity: | Human and Mouse. Does not react with Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody works for FACS, IHC, IP, WB, and ICC. |
| Localization: | Endoplasmic reticulum, outer mitochondrial membrane and intracellular membrane of the nuclear envelope. |
| Control: | Jurkat cell lysate and human breast carcinoma. |
| Background: | The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt(-) |
| ALTnames: | anti- Apoptosis regulator Bcl 2 antibody; anti- Apoptosis regulator Bcl2 antibody ;anti- B cell CLL/lymphoma 2 antibody; anti- B cell leukemia/lymphoma 2 antibody; anti- B cell lymphoma 2 antibody; anti- Bcl 2 antibody; anti- Bcl2 antibody; anti- BCL2 protein antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Hockenbery, D. et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336 (1990). 2. Cleary, M.L. et al. Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation. Cell 47:19-28 (1986). 3. Ruvolo, P.P et al. Phosphorylation of Bcl2 and regulation of apoptosis. Leukemia 15: 515-22 (2001). 4. Deng, X. et al. Novel Role for JNK as a Stress-activated Bcl2 Kinase. J. Biol. Chem. 276: 23681-23688 (2001). |
order or
more information: | company product webpage |
| request information: |
|