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| Catalog Code: | NB110-55486 |
| Product Name: | B-Arrestin-1 Phosphospecific Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho-B-Arrestin-1 (pS412) (E109) [Ser412] |
| Clone: | E109 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser412 of human â-Arrestin-1 was used as immunogen. The antibody only detects â-Arrestin-1 phosphorylated on Serine 412. |
| Cross Reactivity: | Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000IHC: 1:500ICC: 1:100-250IP: 1:100 |
| Background: | â-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). Phosphorylation by Erk1/2 at Serine 412 of the C-terminus is necessary to activate â-arrestin-1 (2). Expanded roles for â-arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot, Immunocytochemistry |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-ARB 1 antibody, anti-ARB1 antibody, anti-ARR 1 antibody, anti-ARR1 antibody, anti-ARRB 1 antibody, anti-ARRB1 antibody, anti-Arrestin beta 1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| Phosphorylated: | Ser412 |
| General References: | 1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677-18680 (1998). 2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971-15974 (1999). 3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640-3 (1998). 4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139-45 (2001). |
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