| CatalogCode: | NB110-55485 |
| ProductName: | B-Arrestin-1 Antibody |
| Product Description: | Rabbit Monoclonal anti-B-Arrestin-1 (E274) |
| Clone: | E274 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues near the N-terminus of Human beta Arrestin 1. |
| Specificity: | A synthetic peptide corresponding to residues near N-terminus of human â-arrestin-1 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology |
| CrossReactivity: | Human, Mouse, and Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500IHC: 1:100ICC: 1:100Flow Cytometry: 1:40 |
| Background: | â-arrestin-1 is a member of the arrestin family that regulates beta-adrenergic receptor function. Beta-arrestins seem to mediate the desensitization and internalization of G protein-coupled receptors (1). Phosphorylation by Erk1/2 at Serine 412 of the C-terminus is necessary to activate â-arrestin-1 (2). Expanded roles for â-arrestin-1 have been studied. Interaction with molecules such as clathrin, AP-2 and NSF directs the clathrin-mediated internalization of G-protein-coupled receptors. Interaction with molecules such as Src, Raf, Erk, ASK1 and JNK3 appears to regulate several pathways that result in the activation of MAP kinases (3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-ARB 1 antibody, anti-ARB1 antibody, anti-ARR 1 antibody, anti-ARR1 antibody, anti-ARRB 1 antibody, anti-ARRB1 antibody, anti-Arrestin beta 1 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Lefkowitz, R.J. G protein-coupled receptors. III. New roles for receptor kinases and beta-arrestins in receptor signaling and desensitization. J. Biol. Chem. 273: 18677-18680 (1998). 2. Lin, F.T., et al. Feedback regulation of beta-arrestin1 function by extracellular signal-regulated kinases. J. Biol. Chem. 274: 15971-15974 (1999). 3. Lin, F.T., et al. beta-arrestins regulate mitogenic signaling and clathrin-mediated endocytosis of the insulin-like growth factor I receptor. J Biol Chem. 273: 31640-3 (1998). 4. Miller, W.E., and R.J. Lefkowitz. Expanding roles for beta-arrestins as scaffolds and adapters in GPCR signaling and trafficking. Curr Opin Cell Biol. 13: 139-45 (2001). |
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