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| Catalog Code: | NB110-55468 |
| Product Name: | ARF1 Antibody |
| Product Description: | Rabbit Monoclonal anti-ARF1 (EP442Y) |
| Clone: | EP442Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.15 mg/ml |
| Immunogen: | A synthetic peptide corresponding to residues in human ARF1. BLAST 'CNTQGLIFVVDSNDRER' |
| Specificity: | A synthetic peptide corresponding to residues in human ARF1 was used as immunogen. This antibody is predicted to detect splice isoforms 3, 4, 5 based on sequence analysis. |
| Cross Reactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml IgG purified Rabbit ascites. |
| Background: | ARF1 (small GTP-binding ADP-ribosylation factor 1) is a member of the ARFs family which contains five other ARF proteins (1). ARF1 functions in various membrane trafficking events in the ER-Golgi system and in the maintenance of organelle structure. Inactive ARF1 (ARF1-GDP) is localizes in the cytoplasm while the active form (ARF1-GTP) is localized in the membrane (2-3). Active ARF1 also localized to the Golgi complex, regulates the assembly of coat proteins onto membranes and mediates traffic in the ER-Golgi. ATF1 activates phospholipase D and phosphatidyllino- sitol 4-phosphate 5-kinase and recruits COPI to membranes (4-5) |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis 1:80, immunohistochemistry 1:250-500, Western Blot 1:2000 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-ADP Ribosylation Factor 1 antibody, anti-ARF 1 antibody, anti-ADP Ribosylation Factor 1 antibody, anti-ARF 1 antibody, anti-ARF1 antibody, anti-DJ133P16.1 antibody, anti-DJ133P16.1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Bobak et al. Proc. Nat. Acad. Sci. 86: 6101-6105, 1989. 2. Serafini, T., Orci, L., Amherdt, M., Brunner, M., Kahn, R. A., and Rothman, J. E. (1991) Cell 67, 239-253 3. Yang, C. Z., Heimberg, H., D'Souza-Schorey, C., Mueckler, M. M., and Stahl, P. D. (1998) J. Biol. Chem. 273, 4006-4011 4. Bonifacino, J.S., and J. Lippincott-Schwartz. 2003. Nat. Rev. Mol. Cell Biol. 4:409-414 5. Nie, Z., D.S. Hirsch, and P.A. Randazzo. 2003. Arf and its many interactors. Curr. Opin. Cell Biol. 15:396-404 |
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