| CatalogCode: | NB110-55463 |
| ProductName: | APAF1 Antibody |
| Product Description: | Rabbit Monoclonal anti-APAF1 (E38) |
| Clone: | E38 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues near N-terminus of human Apaf-1 was used as immunogen. |
| CrossReactivity: | Human, Mouse. Does not react with Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:500IHC: 1:50-100ICC: 1:50-100 |
| Background: | Apaf-1, or apoptotic protease activating factor 1, is a human protein homolog of the C. elagans apoptotic regulatory protein CED-4 that activates caspase-9 during apoptosis (1). Cytosolic Apaf-1 forms an oligomeric complex with caspase-9 in the presence of cytochrome c and dATP, which leads to caspase-9 activation and subsequent activation of caspase-3 (2, 3). Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains, consequently enhancing the catalytic activity of caspase-9 (4), leading to activation of the caspase signaling cascade and apoptosis. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt(-) |
| ALTnames: | anti-APAF 1 antibody, Anti-APAF 1 antibody, Anti-APAF1 antibody, Anti-Apoptotic peptidase activating factor 1 antibody, Anti-Apoptotic protease activating factor antibody, Anti-Apoptotic protease activating factor 1 antibody, Anti-CED 4 antibody, Anti-CED4 antibody, Anti-KIAA0413 antibody, Apoptotic peptidase activating factor 1 antibody, Apoptotic protease activating factor 1 antibody, Apoptotic protease activating factor antibody, CED 4 antibody, CED4 antibody, KIAA0413 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Zou, H. et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90(3): 405-13 (1997). 2. Saleh, A. et al. Cytochrome c and dATP-mediated oligomerization of Apaf-1 is a prerequisite for procaspase-9 activation. J. Biol. Chem. 274 (25): 17941-5 (1999). 3. Hu, Y. et al. Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis. EMBO J. 18(13): 3586-95 (1999). 4. Shiozaki, E.N. et al. Oligomerization and activation of caspase-9, induced by Apaf-1 CARD. PNAS 99(7): 4197-4202 (2002). |
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