| request information: |
|
| Catalog Code: | NB110-55458 |
| Product Name: | AMPK beta 1 Antibody |
| Product Description: | Rabbit Monoclonal anti-AMPK beta 1 (Y367) |
| Clone: | Y367 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues in the human AMPK beta-1 subunit was used as an immunogen. |
| Cross Reactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000-5,000IHC: 1:100-250Flow Cytometry: 1: 50IP: 1:80 |
| Background: | The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1). AMPK is a heterotrimeric protein comprising á (63 kDa), â (38 kDa) and ã (38 kDa) subunits (2). The alpha subunit is the catalytic subunit, while beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (3). AMPK beta-1 mediates the association of the AMPK heterotrimeric complex in vitro (2). Two isoforms have been found and the difference in expression patterns indicates tissue-specific roles for these isoforms (4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis, immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-5 AMP activated protein kinase subunit beta 1 antibody, AMPK antibody, AMPK beta 1 chain antibody, AMPKb antibody, Anti-5 AMP activated protein kinase subunit beta 1, Anti-AMPK, Anti-AMPK beta 1, Anti-AMPK beta 1 chain, Anti-AMPKb, Anti-HAMPKb, Anti-PRKAB1, HAMPKb antibody, PRKAB1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364 2. Woods, A.; Cheung, P. C. F.; Smith, F. C.; Davison, M. D.; Scott, J.; Beri, R. K.; Carling, D.: J. Biol. Chem. 271: 10282-10290, 1996. 3. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136 4. Thornton, C.; Snowden, M. A.; Carling, D.: J. Biol. Chem. 273: 12443-12450, 1998. |
order or
more information: | company product webpage |
|