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| Catalog Code: | NB110-55457 |
| Product Name: | AMPK alpha 1 Antibody |
| Product Description: | Rabbit Monoclonal anti-AMPK alpha 1 (Y365) |
| Clone: | Y365 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Specificity: | A synthetic peptide corresponding to residues near the C-term of human AMPK alpha-1 subunit was used as an immunogen |
| Cross Reactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:1,000-5,000IHC: 1:100-250IP: 1:50 |
| Background: | The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising á (63 kDa), â (38 kDa) and ã (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA |
| Application Summary: | immunohistochemistry, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-5 AMP activated protein kinase catalytic alpha 1 chain antibody, 5' AMP activated protein kinase catalytic subunit alpha 1 antibody, AAPK1 antibody, AMPK 1 antibody, AMPK alpha 1 chain antibody, AMPK antibody, AMPK1 antibody, AMPKa1 antibody, Anti-5 AMP activated protein kinase catalytic alpha 1 chain, Anti-5' AMP activated protein kinase catalytic subunit alpha 1, Anti-AAPK1, Anti-AMPK, Anti-AMPK 1, Anti-AMPK alpha 1, Anti-AMPK alpha 1 chain, Anti-AMPK1, Anti-AMPKa1, Anti-HMG CoA reductase kinase, Anti-MGC33776, Anti-MGC57364, Anti-PRKAA 1, Anti-PRKAA1, Anti-Protein kinase AMP activated alpha 1 catalytic subunit, Anti-acetyl CoA carboxylase kinase, Anti-hormone sensitive lipase kinase, Anti-kinase AMPK alpha1, HMG CoA reductase kinase antibody, MGC33776 antibody, MGC57364 antibody, PRKAA 1 antibody, PRKAA1 antibody, Protein kinase AMP activated alpha 1 catalytic subunit antibody, acetyl CoA carboxylase kinase antibody, hormone sensitive lipase kinase antibody, kinase AMPK alpha1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Mitchelhill, K. I., Stapleton, D., Gao, G., House, C., Michell, B., Katsis, F., Witters, L. A., and Kemp, B. E. (1994) J. Biol. Chem. 269, 2361-2364 2. Stapleton et al. J. Biol. Chem. 271:611-614 3. Hamilton et al. FEBES Lett. 500:163-168, 2001 4. Carling, D., Clarke, P. R., Zammit, V. A., and Hardie, D. G. (1989) Eur. J. Biochem. 186, 129-136 |
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