| CatalogCode: | NB110-55448 |
| ProductName: | AKt1/PKBa Antibody |
| Product Description: | Rabbit Monoclonal anti-AKt1/PKBa (Y89) |
| Clone: | Y89 |
| Clonality: | Monoclonal |
| Specificity: | A synthetic peptide corresponding to residues in C-terminus of human Akt1 was used as immunogen. Predicted to cross-react with bovine based on sequence homology. |
| CrossReactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | WB: 1:5,000-10,000IHC: 1:100ICC: 1:100-250Flow Cytometry: 1:20IP: 1:100 |
| Background: | Akt1/PKBá is a Ser/Thr protein kinase whose kinase activity plays a key role in various cellular functions, including apoptosis, glycogen synthesis, and cell growth . Akt1/PKBá is activated by various growth and survival factors (1). Phosphorylation on Thr308, Ser473 and Tyr474 is required for full activity. Akt1/PKBá inhibits apoptosis by phosphorylating and inactivating several targets, including caspase-9 (2). Akt1/PKBá regulates glycogen synthesis by phosphorylating and inactivating glycogen synthase kinase-3a and b (3,4). Three mammalian Akt1/PKBá isoforms have been identified. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | Anti-AKT1 antibody, anti-C AKT antibody, anti-MGC9965 antibody, anti-Oncogene AKT1 antibody, anti-PKB antibody, anti-PRKBA antibody, anti-Protein Kinase B Alpha antibody, anti-RAC antibody, anti-RAC Alpha antibody, anti-RAC PK Alpha antibody, anti-RAC Serine/Threonine Protein Kinase antibody, anti-vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody, anti-C AKT antibody, anti-MGC9965 antibody, anti-Oncogene AKT1 antibody, anti-PKB antibody, anti-PRKBA antibody, anti-Protein Kinase B Alpha antibody, anti-RAC Alpha antibody, anti-RAC PK Alpha antibody, anti-RAC Serine/Threonine Protein Kinase antibody, anti-RAC antibody, anti-vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Burgering, B.M. and P.J. Coffer. Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376: 599-602 (1995). 2. Cardone, M.H., et al. Regulation of cell death protease caspase-9 by phosphorylation. Science 282: 1318-1321 (1998). 3. Hajduch, E., et al. Protein kinase B (PKB/Akt)--a key regulator of glucose transport? FEBS Lett. 492: 199-203 (2001). 4. Cross, D.A., et al. Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B. Nature 378: 785-789 (1995). |
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