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| Catalog Code: | NB110-55446 |
| Product Name: | AIF Antibody |
| Product Description: | Rabbit Monoclonal anti-AIF (E20) |
| Clone: | E20 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | A synthetic peptide correspndonding to residues near the C terminus of Human AIF. |
| Specificity: | A synthetic peptide corresponding to residues near C-terminus of human AIF was used as an immunogen. |
| Cross Reactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Background: | AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis 1:50, immunohistochemistry 1:500, immunoprecipitation 1:10, Western Blot 1:1,000, Immunocytochemistry 1:500, |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-AIF, Anti-Apoptosis inducing factor, Anti-Harlequin, Anti-Hq, Anti-MGC111425, Anti-MGC5706, Anti-PDCD 8, Anti-PDCD8, Anti-Programmed cell death 8, Anti-Programmed cell death 8 isoform 1, Anti-Programmed cell death 8 isoform 2, Anti-Programmed cell death 8 isoform 3, Anti-Programmed cell death protein 8 mitochondrial, Anti-Programmed cell death protein 8 mitochondrial precursor, Anti-Striatal apoptosis inducing factor, Anti-mAIF, Apoptosis inducing factor antibody, Harlequin antibody, Hq antibody, MGC111425 antibody, MGC5706 antibody, PDCD 8 antibody, PDCD8 antibody, Programmed cell death 8 antibody, Programmed cell death 8 isoform 1 antibody, Programmed cell death 8 isoform 2 antibody, Programmed cell death 8 isoform 3 antibody, Programmed cell death protein 8 mitochondrial antibody, Programmed cell death protein 8 mitochondrial precursor antibody, Striatal apoptosis inducing factor antibody, mAIF antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Susin, S.A. et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 397: 441-6 (1999). 2. Daugas, E. et al. Apoptosis-inducing factor (AIF): a ubiquitous mitochondrial oxidoreductase involved in apoptosis. FEBS Lett. 476: 118-23 (2000). 3. Daugas, E. et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J. 14:729-39 (2000). 4. Susin, S.A. et al. Two distinct pathways leading to nuclear apoptosis. J. Exp. Med. 192: 571-80 (2000). |
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