| CatalogCode: | NB110-55443 |
| ProductName: | Phospho Adducin alpha (pS481) Antibody |
| Product Description: | Rabbit Monoclonal anti-Phospho Adducin alpha (pS481) (EP733Y) |
| Clone: | EP733Y |
| Clonality: | Monoclonal |
| Specificity: | A synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin was used as an immunogen. The antibody only detects alpha Adducin phosphorylated on Serine 481. |
| CrossReactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: 1:20,000IHC: 1:250 - 500ICC: 1:100 -250IP: 1:50 |
| Background: | Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; á and â, or á and ã subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| ListPrice: | 325 |
| AppSummary: | IHC, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-ADD 1 antibody, anti-ADD A antibody, anti-ADD1 antibody, anti-ADD1 protein antibody, anti-ADDA antibody, anti-Adducin 1 (alpha) antibody, anti-Adducin 1 alpha antibody, anti-Alpha adducin antibody, anti-ADD 1, anti-ADD A, anti-ADD1, anti-ADD1 protein, anti-ADDA, anti-Adducin 1 (alpha), anti-Adducin 1 alpha, anti-Erythrocyte adducin alpha subunit, anti-Erythrocyte adducin subunit alpha, anti-MGC3339, anti-MGC44427, anti-alpha Adducin, anti-Erythrocyte adducin alpha subunit antibody, anti-Erythrocyte adducin subunit alpha antibody, anti-MGC3339 antibody, anti-MGC44427 antibody |
| PackageSize: | 0.1 ml |
| GeneralRef: | 1. Gardner K, Bennett V., J Biol Chem 261:1339-1348, 1986. 2. Kuhlman PE, et al. J Biol. Chem, 271: 7986-7991, 1996. 3. Dong, LC, et al. J Biol Chem 270: 25534-25540, 1995. 4. Palfrey HC, et al. J Biol Chem. 260:16021-16029, 1985. 5. Fukata, et al. J Cell Biol, 145:347-361, 1995. |
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