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| Catalog Code: | NB110-55438 |
| Product Name: | ABCG1 Antibody |
| Product Description: | Rabbit Monoclonal anti-ABCG1 (EP1366Y) |
| Clone: | EP1366Y |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | A synthetic peptide corresponding to residues on human ABCG1. |
| Specificity: | A synthetic peptide corresponding to residues on human ABCG1 was used as an immunogen. |
| Cross Reactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml Tissue culture supernatant Rabbit ascites. |
| Uses: | This antibody can be used in WB, IHC and ICC. |
| Localization: | Cell Membrane and Cytoplasmic |
| Background: | ATP-binding cassette (ABC) transporter ABCG1 (ABC8) is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. Conversely, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1. Immunocytochemical and flow cytometric analyses revealed that ABCG1 is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages. Results demonstrate a regulator function for ABCG1 in cholesterol and phospholipid transport define a biologic activity for ABC transporters in macrophages (1). ABC transporters belonging to the ABCG (White) subfamily (ABCG1, ABCG5, and ABCG8) have been shown to be critically involved in the regulation of lipid-trafficking mechanisms in macrophages, hepatocytes, and intestinal mucosa cells (2) Studies show that treatment with Rhodobacter sphaeroiders LPS, an LPS antagonist, or PD169316, a specific p38 MAP kinase inhibitor, prevented the induction of ABCA1 by LPS. Therefore, this suggests that both transport of LPS from the plasma membrane to an intracellular site and activation of p38 MAP kinase are involved in the LPS-mediated induction of ABCA1 (3). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | immunohistochemistry 1:50 - 100, Western Blot 1:2000, Immunocytochemistry 1:100 - 250 |
| Species Summary: | Bv, Hu, Mu, Rt, |
| Alternate Names: | anti-Abc8 antibody, anti-ATP-binding cassette sub-family G member 1 antibody, anti-ATP-binding cassette transporter 8 antibody, anti-White protein homolog antibody, anti-wht1 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Klucken J, et al. Proc Natl Acad Sci 97(2):817-22, 2000 2. Kaplan R, et al. J Lipid Res 43(6):952-9, 2002. 3. Schmitz G, et al. J Lipid Res 42(10):1513-20, 2001 |
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