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| Catalog Code: | NB110-55432 |
| Product Name: | a-Actin (Smooth Muscle) Antibody |
| Product Description: | Rabbit Monoclonal anti-a-Actin (Smooth Muscle) (E184) |
| Clone: | E184 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide corresponding to N-terminus (Human) |
| Specificity: | A synthetic peptide corresponding to N-terminus of human Alpha-actin 3 was used as immunogen. This antibody only detects actin in smooth muscle in immunohistochemistry. |
| Cross Reactivity: | Human, Mouse, Rat. Not tested in other species. |
| Packaging: | 0.1 ml IgG purified Rabbit ascites. |
| Uses: | WB: 1:50,000IHC: 1:500Flow Cytometry: 1:20IP: 1:20 |
| Background: | Actins are highly conserved proteins ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility (1). Polymerization of globular actin (G-actin) leads to a structural filament (F-actin), whose formation requires the Arp2/3 complex (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). It has also been shown that actin dysfunction leads to certain types of heart failure (4). Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Furthermore, expression of smooth muscle alpha actin is regulated by hormones and cell proliferation, and is altered by pathological conditions including oncogenic transformation and atherosclerosis (5). |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Application Summary: | flow cytometry / FACS analysis , immunohistochemistry , immunoprecipitation , Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-ACTA2 antibody, anti-ACTSA antibody, anti-ACTVS antibody, anti-Alpha-actin 2 antibody, anti-ACTA2 antibody, anti-ACTSA antibody, anti-ACTVS antibody, anti-Alpha-actin 2 antibody, anti-alpha smooth muscle Actin antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.01% sodium azide |
| General References: | 1. Herman, I.M. Actin isoforms. Curr. Opin. Cell Biol. 5: 48-55 (1993). 2. Bailly, M., et al. The F-actin side binding activity of the Arp2/3 complex is essential for actin nucleation and lamellipod extension. Curr Biol. 11: 620-5 (2001). 3. Lim, Y.P., et al. Selective tyrosine hyperphosphorylation of cytoskeletal and stress proteins in primary human breast cancers: implications for adjuvant use of kinase-inhibitory drugs. Clin. Cancer Res. 10: 3980-3987 (2004). 4. Olson, T.M., et al. Actin mutations in dilated cardiomyopathy, a heritable form of heart failure. Science 280: 750-2 (1998). 5. Miwa, T., et al. Structure, chromosome location, and expression of the human smooth muscle (enteric type) gamma-actin gene: evolution of six human actin genes. Mol Cell Biol. 11: 3296-306 (1991). |
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