| CatalogCode: | NB110-55384 |
| ProductName: | Beta-Tubulin Antibody |
| Product Description: | Mouse Monoclonal anti-Beta-Tubulin (TU-13) |
| Clone: | TU-13 |
| Clonality: | Monoclonal |
| Immunogen: | beta-tubulin from porcine brain |
| Specificity: | The antibody TU-13 recognizes N-terminal structural domain of beta-Tubulin, a 50 kDa intracellular structural constituent of microtubules. The beta-tubulins are primary localizated in cytoplasm. |
| CrossReactivity: | Human, Mouse, Plants. Not tested in other species. |
| Packaging: | 0.1 mg peptide affinity purified Mouse ascites. |
| Uses: | Western BlottingImmunohistochemistry (frozen sections)ImmunocytochemistryELISA |
| Background: | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening - this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. |
| Isotype: | IgM |
| Host_Name: | Mouse |
| Buffer: | Phosphate buffered saline (PBS), approx. pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | ELISA, IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Pl |
| PackageSize: | 0.1 mg |
| GeneralRef: | Linhartova I, Draberova E, Viklicky V, Draber P.: Distribution of non-class-III beta-tubulin isoforms in neuronal and non-neuronal cells. FEBS Lett. 1993 Mar 29;320(1):79-82. Lewis, S.A., Cowan, N.J.: Tubulin genes: structure, expression, and regulation. In: Avila, J. (ed.): Microtubule proteins. Boca Raton: CRC Press, Inc. 1990. Pp. 37-66. Draber P, Lagunowich LA, Draberova E, Viklicky VDamjanov I.: Heterogeneity of tubulin epitopes in mouse fetal tissues. Histochemistry. 1988;89(5):485-92. Draber P, Vater W, Bohm KJ, Kuklova E, Unger E.: Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies. FEBS Lett. 1990 Mar 26;262(2):209-11. |
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